erk pathway inhibitor pd98059  (MedChemExpress)

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibitor suppresses p-TrkA-mediated upregulation of ERK, EGR1, and p35 in the MPC5 cells grown under HG conditions. Representative western blots show the levels of (A) TrkA and phospho-TrkA (Tyr490), (C) ERK, phospho-ERK, and EGR1, and (E) CDK5 and p35 in the LG, HG+Vehicle (0.1% DMSO), HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups. Representative western blots show the levels of (B) TrkA and phospho-TrkA (Tyr490), (D) ERK, phospho-ERK, and EGR1, and (F) CDK5 and p35 in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p < 0.05 was considered statistically significant.

Article Snippet: The ERK pathway inhibitor PD98059 (HY-12028, MedChemExpress, CA, USA).

Techniques: Western Blot, Plasmid Preparation

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition alleviates inflammation and injury in the HG-stimulated MPC5 cells. Representative western blots show the expression levels of (A) TNF-α and IL-1β, and (G) synaptopodin in the LG, HG+Vehicle, HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups. Representative western blots show the expression levels of (D) TNF-α; IL-1β, and (I) synaptopodin in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. Quantitative analysis shows the relative gene expression levels of (B) TNF-α; (C) IL-6; and (H) Nephrin in the LG, HG+Vehicle, LG + Man, HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups. (E) TNF-α; (F) IL-1β; and (J) Nephrin in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p < 0.05 was considered statistically significant.

Article Snippet: The ERK pathway inhibitor PD98059 (HY-12028, MedChemExpress, CA, USA).

Techniques: Inhibition, Western Blot, Expressing, Plasmid Preparation, Gene Expression

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition alleviates apoptosis in the HG-stimulated MPC5 cells. (A) Flow cytometry plots show the analysis of the percentage of apoptotic cells in the LG, HG+Vehicle, HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups based on Annexin V/PI double staining. PI distinguishes viable cells from apoptotic cells; Annexin V-FITC specifically identifies apoptotic cells. (B) Quantitative analysis of the percent apoptotic cells in different groups based on data shown in (A) . (C) The analysis of the percentage of apoptotic cells in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. (D) Quantitative analysis of the percent apoptotic cells in different groups based on data shown in (C) . *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p < 0.05 was considered statistically significant.

Article Snippet: The ERK pathway inhibitor PD98059 (HY-12028, MedChemExpress, CA, USA).

Techniques: Inhibition, Flow Cytometry, Plasmid Preparation, Double Staining

Journal: Viruses

Article Title: Unveiling the Antiviral Potential of Minocycline: Modulation of Nuclear Export of Viral Ribonuclear Proteins during Influenza Virus Infection

doi: 10.3390/v16081317

Figure Lengend Snippet: ( A ) IAV/PR8-infected MDCK cells treated with minocycline (500 nM) or PD98059 (30 µM) and incubated for 18 and 24 hpi. The cells were then fixed, permeabilized, and stained with anti-NP antibodies (raised in mice). The cells were then secondarily stained with DyLight488-labeled anti-mouse secondary antibody. The cells were mounted using DAPI and visualized under a confocal microscope (63X oil immersion). Scale bar: 20 µm. ( B ) Whole-cell, nuclear, and cytosolic fractions of IAV/PR8-infected cells treated with minocycline (500 nM) or PD98059 (30 µM) at 6 hpi and 24 hpi were extracted. A Western blot was performed with β-actin serving as the loading control for cytosolic and whole-cell lysate and lamin A/C for nuclear fractions. ( C ) Proteins from PMA-induced MDCK cells at 18 hpi were extracted and Western blot analysis was performed to observe phosphorylation of ERK, with β-actin serving as the loading control. ( D ) Western blot analysis of IAV/PR8-infected MDCK cells treated with minocycline (500 nM) was conducted to observe phosphorylation of ERK at 24 hpi. ( E ) HEK293T cells were transfected with only pcDNA3 vector and pcDNA3-HA plasmid and treated with 500 nM of minocycline for 24 h; the cells were lysed and a Western blot was performed to detect the level of phospho-ERK.

Article Snippet: MEK/ERK pathway inhibitor PD98059 (M2865-17A.1500) was purchased from Biomol.

Techniques: Infection, Incubation, Staining, Labeling, Microscopy, Western Blot, Control, Phospho-proteomics, Transfection, Plasmid Preparation